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Immunologie et infection
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Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
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Immunologie et infection
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Journal JoVE Immunologie et infection
Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

7,960 Views

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09:44 min

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June 13, 2018

DOI:

10.3791/57651-v

DOI:
10.3791/57651-v

•

09:44 min
June 13, 2018

•

4 Views
, , ,
1Department of Biophysics, Center for Integrative Physiology and Molecular Medicine (CIPMM), School of Medicine,Saarland University

Summary

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Here, we present a protocol to visualize immune cells embedded in a three-dimensional (3D) collagen matrix using light-sheet microscopy. This protocol also elaborates how to track cell migration in 3D. This protocol can be employed for other types of suspension cells in the 3D matrix.

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Light-sheet MicroscopyThree-dimensional VisualizationHuman Immune CellsImmunologyCell BehaviorPhysiologically Relevant ContextCollagenFluorescent LabelingCell CultureCapillaryImaging

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Cite this Article

Schoppmeyer, R., Zhao, R., Hoth, M., Qu, B. Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells. J. Vis. Exp. (136), e57651, doi:10.3791/57651 (2018).

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