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Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells
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JoVE Journal 免疫学と感染
Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells

DOI:
10.3791/57651-v

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09:44 min

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June 13, 2018

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, , ,
1Department of Biophysics, Center for Integrative Physiology and Molecular Medicine (CIPMM), School of Medicine,Saarland University

章

  • 00:04標題
  • 00:53Preparation of Neutralized Collagen
  • 01:55Sample Preparation for Light-sheet Fluorescence Microscopy Using Capillaries
  • 04:08Image Acquisition Using Light-sheet Microscopy
  • 05:07Fixation and Immunofluorescence Staining of Cells in Collagen Matrices
  • 07:17Results: Visualization of Human CTL Cells by Light-sheet Microscopy
  • 08:32Conclusion

概要

自動翻訳

自動翻訳

Here, we present a protocol to visualize immune cells embedded in a three-dimensional (3D) collagen matrix using light-sheet microscopy. This protocol also elaborates how to track cell migration in 3D. This protocol can be employed for other types of suspension cells in the 3D matrix.

タグ

Light-sheet MicroscopyThree-dimensional VisualizationHuman Immune Cells免疫学Cell BehaviorPhysiologically Relevant ContextCollagenFluorescent LabelingCell CultureCapillaryImaging

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