Journal
/
신경과학
/
Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

DOI:
10.3791/57627-v

•

10:44 min

•

June 20, 2018

•

, , ,
1UPMC Univ Paris 06, INSERM, CNRS, Neuroscience Paris Seine – Institut de Biologie Paris Seine (NPS – IBPS),Sorbonne Université

Chapters

  • 00:04Title
  • 00:57Acute Slice Preparation
  • 02:53Single Cell RT-PCR after Patch-clamp Recording
  • 05:54PCR Validation
  • 08:47Results: Characterization of Layer V Neocortical Pyramidal Cells
  • 09:57Conclusion

Summary

자동 번역

자동 번역

This protocol describes the critical steps and precautions required to perform single cell multiplex reverse transcription polymerase chain reaction after patch-clamp. This technique is a simple and effective method to analyze the expression profile of a predetermined set of genes from a single cell characterized by patch-clamp recordings.

Tags

Keywords: Single CellMultiplexReverse TranscriptionPolymerase Chain ReactionPatch-clamp신경과학Neuronal PopulationTransmission SystemsGene ExpressionAstrocytesAnimal ModelsBrain DissectionVibratome SectioningWhole-cell RecordingCytoplasm HarvestingRT-PCRGDNA Contamination

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/57627?article_type=v

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices
check_url/kr/57627?article_type=v

Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation
check_url/kr/57627?article_type=v

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells
check_url/kr/57627?article_type=v

A Computer-assisted Multi-electrode Patch-clamp System
check_url/kr/57627?article_type=v

Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons
check_url/kr/57627?article_type=v

Whole-cell Patch-clamp Recordings in Brain Slices
check_url/kr/57627?article_type=v

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins
check_url/kr/57627?article_type=v

Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity
check_url/kr/57627?article_type=v

Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats
check_url/kr/57627?article_type=v

Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons

Read Article