Journal
/
Neurociência
/
Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

9,536 Views

•

10:44 min

•

June 20, 2018

DOI:

10.3791/57627-v

DOI:
10.3791/57627-v

•

10:44 min
June 20, 2018

•

12 Views
, , ,
1UPMC Univ Paris 06, INSERM, CNRS, Neuroscience Paris Seine – Institut de Biologie Paris Seine (NPS – IBPS),Sorbonne Université

Summary

Automatically generated

This protocol describes the critical steps and precautions required to perform single cell multiplex reverse transcription polymerase chain reaction after patch-clamp. This technique is a simple and effective method to analyze the expression profile of a predetermined set of genes from a single cell characterized by patch-clamp recordings.

Explore More Videos

Single CellMultiplexReverse TranscriptionPolymerase Chain ReactionPatch-clampNeurociênciaNeuronal PopulationTransmission SystemsGene ExpressionAstrocytesAnimal ModelsBrain DissectionVibratome SectioningWhole-cell RecordingCytoplasm HarvestingRT-PCRGDNA Contamination

Read Article

Cite this Article

Devienne, G., Le Gac, B., Piquet, J., Cauli, B. Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp. J. Vis. Exp. (136), e57627, doi:10.3791/57627 (2018).

Download .ris file

Copy

Share Video

.

Copy