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Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
 

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Article DOI: 10.3791/57627-v • 10:44 min • June 20th, 2018
June 20th, 2018 •
*1, *1, *1, 1
1UPMC Univ Paris 06, INSERM, CNRS, Neuroscience Paris Seine - Institut de Biologie Paris Seine (NPS - IBPS), Sorbonne Université
* These authors contributed equally

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This protocol describes the critical steps and precautions required to perform single cell multiplex reverse transcription polymerase chain reaction after patch-clamp. This technique is a simple and effective method to analyze the expression profile of a predetermined set of genes from a single cell characterized by patch-clamp recordings.

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