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Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
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神经科学
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JoVE 杂志 神经科学
Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

9,536 Views

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10:44 min

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June 20, 2018

DOI:

10.3791/57627-v

DOI:
10.3791/57627-v

•

10:44 min
June 20, 2018

•

12 Views
, , ,
1UPMC Univ Paris 06, INSERM, CNRS, Neuroscience Paris Seine – Institut de Biologie Paris Seine (NPS – IBPS),Sorbonne Université

Summary

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This protocol describes the critical steps and precautions required to perform single cell multiplex reverse transcription polymerase chain reaction after patch-clamp. This technique is a simple and effective method to analyze the expression profile of a predetermined set of genes from a single cell characterized by patch-clamp recordings.

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Devienne, G., Le Gac, B., Piquet, J., Cauli, B. Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp. J. Vis. Exp. (136), e57627, doi:10.3791/57627 (2018).

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