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成年日本血吸虫细胞外囊泡的分离与鉴定
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum
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成年日本血吸虫细胞外囊泡的分离与鉴定

DOI:
10.3791/57514-v

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07:37 min

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May 22, 2018

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, , , , , ,
1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology,Ministry of Agriculture, 2Tianjin Agricultural University

Chapters

  • 00:04Title
  • 00:46Isolating SjEVs from Schistosoma japonicum Cultures
  • 03:00Characterization of EVs by Western Blotting and TEM
  • 04:01Labeling Schistosoma japonicum Extracellular Vesicles with PKH67 Followed by Cell Uptake
  • 06:15Results: Schistosoma japonicum Extracellular Vesicle Characterization
  • 07:17Conclusion

Summary

자동 번역

자동 번역

在这里, 我们提出一个协议, 以描述细胞外囊泡 (电动车) 隔离在体外培养培养基从成年日本血吸虫。

Tags

Extracellular VesiclesSchistosoma JaponicumIn Vitro CultureIsolationPurificationCentrifugationDialysisPEG8000Western Blot

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