Journal
/
免疫与感染
/
Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

DOI:
10.3791/57514-v

•

07:37 min

•

May 22, 2018

•

, , , , , ,
1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology,Ministry of Agriculture, 2Tianjin Agricultural University

Chapters

  • 00:04Title
  • 00:46Isolating SjEVs from Schistosoma japonicum Cultures
  • 03:00Characterization of EVs by Western Blotting and TEM
  • 04:01Labeling Schistosoma japonicum Extracellular Vesicles with PKH67 Followed by Cell Uptake
  • 06:15Results: Schistosoma japonicum Extracellular Vesicle Characterization
  • 07:17Conclusion

Summary

自动翻译

自动翻译

Here, we present a protocol to describe extracellular vesicles (EVs) isolation in in vitro cultured medium from adult Schistosoma japonicum.

Tags

Extracellular VesiclesSchistosoma JaponicumIn Vitro CultureIsolationPurificationCentrifugationDialysisPEG8000Western Blot

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Cercarial Transformation and in vitro Cultivation of Schistosoma mansoni Schistosomules

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network

Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes

Flow Virometry to Analyze Antigenic Spectra of Virions and Extracellular Vesicles

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies

Read Article