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Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum

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07:37 min

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May 22, 2018

DOI:

10.3791/57514-v

DOI:
10.3791/57514-v

•

07:37 min
May 22, 2018

•

4 Views
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1Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal Parasitology,Ministry of Agriculture, 2Tianjin Agricultural University

Summary

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Here, we present a protocol to describe extracellular vesicles (EVs) isolation in in vitro cultured medium from adult Schistosoma japonicum.

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Extracellular VesiclesSchistosoma JaponicumIn Vitro CultureIsolationPurificationCentrifugationDialysisPEG8000Western Blot

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Cite this Article

Liu, J., Zhu, L., Wang, L., Chen, Y., Giri, B. R., Li, J., Cheng, G. Isolation and Characterization of Extracellular Vesicles from Adult Schistosoma japonicum. J. Vis. Exp. (135), e57514, doi:10.3791/57514 (2018).

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