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二维半变性洗涤剂琼脂糖凝胶电泳法确定淀粉样或淀粉样纤维的粒度异质性

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Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers

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二维半变性洗涤剂琼脂糖凝胶电泳法确定淀粉样或淀粉样纤维的粒度异质性

DOI:

10.3791/57498-v

•

10:10 min

•

April 26, 2018

•

Sarah Hanna-Addams, Zhigao Wang

¹Department of Molecular Biology,UT Southwestern Medical Center

Chapters

00:04Title
00:47Preparation of the Sample
03:22Preparing and Running the Gel
04:30Transfer
06:07Western Blotting Detection
07:45Results: Use of SDD-AGE to Confirm the Presence of Heterogenous Sizes of Amyloid-like Fibers
09:24Conclusion

Summary

자동 번역

在这里, 我们使用二维半变性琼脂糖凝胶电泳, 以确认存在的异质淀粉样纤维的异构大小, 并排除了大小异质性是由于淀粉样纤维在凝胶中离解的可能性运行过程。

Tags

2D Semi-denaturing Detergent Agarose Gel Electrophoresis Amyloid Fibers Size Heterogeneity Protein Aggregation SDD-AGE HT-29 Colon Cancer Cells Cell Culture Cell Lysis Whole Cell Lysate SDD-AGE Buffer

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