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Biochemistry

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JoVE Journal Biochemistry
Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers
 

Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers

Article DOI: 10.3791/57498-v • 10:10 min • April 26th, 2018
April 26th, 2018 •
1, 1
1Department of Molecular Biology, UT Southwestern Medical Center

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Here we use two-dimensional semi-denaturing agarose gel electrophoresis to confirm the presence of amyloid-like fibers of heterogeneous size and exclude the possibility that the size heterogeneity is due to dissociation of the amyloid fibers during the gel running process.

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Two-dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis Size Heterogeneity Amyloid Fibers Amyloid-like Fibers SDD-AGE Protein Aggregation Protein Degradation Dissociation Lab Technique Specialized Equipment HT-29 Colon Cancer Cells Tissue Culture Dish Phosphate-buffered Saline Trypsin Culture Medium Centrifuge
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