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Manipulation of Ploidy in Caenorhabditis elegans
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Manipulation of Ploidy in Caenorhabditis elegans

Manipulation of Ploidy in Caenorhabditis elegans

10,578 Views

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07:54 min

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March 15, 2018

DOI:

10.3791/57296-v

DOI:
10.3791/57296-v

•

07:54 min
March 15, 2018

•

10 Views
, , , ,
1Brooklyn College, Biology Department,City University of New York, 2The Graduate Center, Biology Department,City University of New York, 3Advanced Science Research Center,City University of New York

Summary

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This method allows for the generation of tetraploid and triploid Caenorhabditis nematodes from any diploid strain. Polyploid strains generated by this method have been used to study chromosome interactions in meiotic prophase, and this method is useful for examining important basic questions in cell, developmental, evolutionary, and cancer biology.

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Clarke, E. K., Rivera Gomez, K. A., Mustachi, Z., Murph, M. C., Schvarzstein, M. Manipulation of Ploidy in Caenorhabditis elegans. J. Vis. Exp. (133), e57296, doi:10.3791/57296 (2018).

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