Manipulation of Ploidy in <em>Caenorhabditis elegans</em>

Erlyana K. Clarke; Katherine A. Rivera Gomez; Zaki Mustachi; Mikaela C. Murph; Mara Schvarzstein

doi:10.3791/57296

Journal

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Génétique

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Manipulation of Ploidy in Caenorhabditis elegans

Journal JoVE
Génétique

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Journal JoVE Génétique

Manipulation of Ploidy in Caenorhabditis elegans

Manipulation of Ploidy in Caenorhabditis elegans

10,633 Views

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07:54 min

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March 15, 2018

DOI:

10.3791/57296-v

DOI:

10.3791/57296-v

•

07:54 min

March 15, 2018

•

10 Views

Erlyana K. Clarke¹, Katherine A. Rivera Gomez^1,2, Zaki Mustachi¹, Mikaela C. Murph^1,3, Mara Schvarzstein^1,2,3

¹Brooklyn College, Biology Department,City University of New York, ²The Graduate Center, Biology Department,City University of New York, ³Advanced Science Research Center,City University of New York

Summary

This method allows for the generation of tetraploid and triploid Caenorhabditis nematodes from any diploid strain. Polyploid strains generated by this method have been used to study chromosome interactions in meiotic prophase, and this method is useful for examining important basic questions in cell, developmental, evolutionary, and cancer biology.

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Manipulation of Ploidy in Caenorhabditis elegans

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