Journal
/
遗传学
/
Manipulation of Ploidy in Caenorhabditis elegans
JoVE 杂志
遗传学
This content is Free Access.
JoVE 杂志 遗传学
Manipulation of Ploidy in Caenorhabditis elegans

Manipulation of Ploidy in Caenorhabditis elegans

10,633 Views

•

07:54 min

•

March 15, 2018

DOI:

10.3791/57296-v

DOI:
10.3791/57296-v

•

07:54 min
March 15, 2018

•

10 Views
, , , ,
1Brooklyn College, Biology Department,City University of New York, 2The Graduate Center, Biology Department,City University of New York, 3Advanced Science Research Center,City University of New York

Summary

Automatically generated

This method allows for the generation of tetraploid and triploid Caenorhabditis nematodes from any diploid strain. Polyploid strains generated by this method have been used to study chromosome interactions in meiotic prophase, and this method is useful for examining important basic questions in cell, developmental, evolutionary, and cancer biology.

Explore More Videos

TetraploidPloidyCaenorhabditis ElegansRec-8RNAiPolyploidizationGene DosageGenome InstabilitySpeciationDiploidSpermatocytesOocytesE. ColiIPTGAmpicillinLBHermaphroditesL4 Stage

Read Article

Cite this Article

Clarke, E. K., Rivera Gomez, K. A., Mustachi, Z., Murph, M. C., Schvarzstein, M. Manipulation of Ploidy in Caenorhabditis elegans. J. Vis. Exp. (133), e57296, doi:10.3791/57296 (2018).

Download .ris file

Copy

Share Video

.

Copy