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A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
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A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

10,516 Views

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10:27 min

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April 20, 2018

DOI:

10.3791/57192-v

DOI:
10.3791/57192-v

•

10:27 min
April 20, 2018

•

8 Views
, , , , , ,
1College of Life Sciences,Guangzhou University, 2Hard Winter Wheat Genetics Research Unit,United States Department of Agriculture – Agricultural Research Service, 3The UWA Institute of Agriculture,University of Western Australia

Summary

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Here, we report a simple and low-cost silver staining protocol which requires only three reagents and 7 min of processing, and is suitable for fast generation of high-quality SSR data in the genetic analysis.

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Silver StainingSSR MarkersNon-denaturing Polyacrylamide GelGenetic ResearchMarker-assisted SelectionMolecular BreedingRapid GenotypingEasyLow CostOptimized ProtocolBind-silaneRepel-silaneTEMEDAPSElectrophoresis

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Huang, L., Deng, X., Li, R., Xia, Y., Bai, G., Siddique, K. H., Guo, P. A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel. J. Vis. Exp. (134), e57192, doi:10.3791/57192 (2018).

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