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A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
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Genetica
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JoVE Journal Genetica
A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

DOI:
10.3791/57192-v

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10:27 min

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April 20, 2018

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, , , , , ,
1College of Life Sciences,Guangzhou University, 2Hard Winter Wheat Genetics Research Unit,United States Department of Agriculture – Agricultural Research Service, 3The UWA Institute of Agriculture,University of Western Australia

Capitoli

  • 00:04Titolo
  • 01:38Preparation of Polyacrylamide Gels for Electrophoresis
  • 04:03Running Gels and Silver Staining for Detecting SSR Markers in a Non-polyacrylamide Gel
  • 07:43Results: Fast Silver Staining to Detect SSR Markers on a Non-denaturing Polyacrylamide Gel
  • 09:18Conclusion

Summary

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Here, we report a simple and low-cost silver staining protocol which requires only three reagents and 7 min of processing, and is suitable for fast generation of high-quality SSR data in the genetic analysis.

Tags

Silver StainingSSR MarkersNon-denaturing Polyacrylamide GelGenetic ResearchMarker-assisted SelectionMolecular BreedingRapid GenotypingEasyLow CostOptimized ProtocolBind-silaneRepel-silaneTEMEDAPSElectrophoresis

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