Journal
/
Genetik
/
A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
JoVE Journal
Genetik
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Genetik
A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

DOI:
10.3791/57192-v

•

10:27 min

•

April 20, 2018

•

, , , , , ,
1College of Life Sciences,Guangzhou University, 2Hard Winter Wheat Genetics Research Unit,United States Department of Agriculture – Agricultural Research Service, 3The UWA Institute of Agriculture,University of Western Australia

Kapitel

  • 00:04Titel
  • 01:38Preparation of Polyacrylamide Gels for Electrophoresis
  • 04:03Running Gels and Silver Staining for Detecting SSR Markers in a Non-polyacrylamide Gel
  • 07:43Results: Fast Silver Staining to Detect SSR Markers on a Non-denaturing Polyacrylamide Gel
  • 09:18Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we report a simple and low-cost silver staining protocol which requires only three reagents and 7 min of processing, and is suitable for fast generation of high-quality SSR data in the genetic analysis.

Tags

Silver StainingSSR MarkersNon-denaturing Polyacrylamide GelGenetic ResearchMarker-assisted SelectionMolecular BreedingRapid GenotypingEasyLow CostOptimized ProtocolBind-silaneRepel-silaneTEMEDAPSElectrophoresis

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Detection of Copy Number Alterations Using Single Cell Sequencing

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression

Read Article