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JoVE Journal Genetics
Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells
 

Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

Article DOI: 10.3791/57140-v • 09:27 min • June 1st, 2018
June 1st, 2018 •
1,2, 1, 1, 1, 1, 3, 3, 1,2
1Department of Genetics, Texas Biomedical Research Institute, 2Department of Internal Medicine-Molecular Medicine, Wake Forest University School of Medicine, 3Department of Chemistry, University of Wisconsin

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요약
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This is a method to identify novel DNA-interacting proteins at specific target loci, relying on sequence-specific capture of crosslinked chromatin for subsequent proteomic analyses. No prior knowledge about potential binding proteins, nor cell modifications are required. Initially developed for yeast, the technology has now been adapted for mammalian cells.

Tags

Hybridization Capture Chromatin-associated Proteins Proteomics Mammalian Cells Genome Regulation Disease-associated Sequence Variants Gene Expression Protein Binding DNA Binding Proteins Oligonucleotides Lymphoblastoid Cells RPMI 1640 Medium Cell Density
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