ジャーナル
/
/
Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells
JoVE Journal
遺伝学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 遺伝学
Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

6,098 Views

09:27 min

June 01, 2018

DOI:

09:27 min
June 01, 2018

1 Views
, , , , , , ,

概要

Automatically generated

This is a method to identify novel DNA-interacting proteins at specific target loci, relying on sequence-specific capture of crosslinked chromatin for subsequent proteomic analyses. No prior knowledge about potential binding proteins, nor cell modifications are required. Initially developed for yeast, the technology has now been adapted for mammalian cells.

Read Article