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Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells
JoVE Journal
Genetics
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JoVE Journal Genetics
Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

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09:27 min

June 01, 2018

DOI:

09:27 min
June 01, 2018

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Summary

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This is a method to identify novel DNA-interacting proteins at specific target loci, relying on sequence-specific capture of crosslinked chromatin for subsequent proteomic analyses. No prior knowledge about potential binding proteins, nor cell modifications are required. Initially developed for yeast, the technology has now been adapted for mammalian cells.

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