JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
18,898 Views
•
10:21 min
•
February 16, 2018
DOI:
Summary
Automatically generated
A protocol for the isolation of primary microglia from murine brains is presented. This technique aids in furthering the current understanding of neurological conditions. Density gradient centrifugation and magnetic separation are combined to produce sufficient yield of a highly pure sample. Furthermore, we outline the steps for characterization of microglia.
Read Article
Cite this Article
Stark, J. C., Wallace, E., Lim, R., Leaw, B. Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation. J. Vis. Exp. (132), e57065, doi:10.3791/57065 (2018).
Copy