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Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

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10:21 min

February 16, 2018

DOI:

10:21 min
February 16, 2018

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Summary

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A protocol for the isolation of primary microglia from murine brains is presented. This technique aids in furthering the current understanding of neurological conditions. Density gradient centrifugation and magnetic separation are combined to produce sufficient yield of a highly pure sample. Furthermore, we outline the steps for characterization of microglia.

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