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Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
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Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation
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February 16, 2018
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A protocol for the isolation of primary microglia from murine brains is presented. This technique aids in furthering the current understanding of neurological conditions. Density gradient centrifugation and magnetic separation are combined to produce sufficient yield of a highly pure sample. Furthermore, we outline the steps for characterization of microglia.
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Stark, J. C., Wallace, E., Lim, R., Leaw, B. Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation. J. Vis. Exp. (132), e57065, doi:10.3791/57065 (2018).
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