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毛细管电聚焦法对蛋白质及其亚型的高灵敏度和定量检测
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JoVE 신문 생화학
Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
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毛细管电聚焦法对蛋白质及其亚型的高灵敏度和定量检测

DOI:
10.3791/56794-v

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07:58 min

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September 19, 2018

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1Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory,751 85, Uppsala, Sweden

Chapters

  • 00:04Title
  • 01:00Sample Mix Preparation (Calculation for 150 L Sample Mix)
  • 03:03Designing a New Assay Template with System Software
  • 04:33Running the Assay File
  • 05:20Analyze the Data
  • 05:46Results: Characterization of Extracellular Signal Regulated Kinase Proteins by Capillary Isoelectric Focusing
  • 07:08Conclusion

Summary

자동 번역

자동 번역

毛细管等电聚焦是一种基于抗体的、超灵敏的高通量技术, 它能够从极小的生物样品中详细描述蛋白质及其亚型。下面介绍了一种自动和机器人的方法, 用于检测和量化特定蛋白质及其亚型。

Tags

Capillary Isoelectric FocusingProtein DetectionProtein IsoformsBiomarker DiscoveryDrug DiscoveryDiagnosticsHigh-throughputReproducibleCell-signaling PathwaysProtein AnalysisSample PreparationIsoelectric Point Standard LadderAntibody DilutionLuminol-peroxide Detection

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