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タンパク質キャピラリー等電点焦点法によるアイソフォームの高感度・定量的検出

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Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

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タンパク質キャピラリー等電点焦点法によるアイソフォームの高感度・定量的検出

6,431 Views

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07:58 min

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September 19, 2018

DOI:

10.3791/56794-v

DOI:

10.3791/56794-v

•

07:58 min

September 19, 2018

•

9 Views

Narendra Padhan¹

¹Uppsala University, Dept. Immunology, Genetics and Pathology, Rudbeck Laboratory,751 85, Uppsala, Sweden

Summary

キャピラリー等電集中はタンパク質と非常に小さい試料からアイソフォームの詳細な特性解析を有効に、抗体を用いた、超高感度、高スループット手法です。次に、自動化・ロボット化の方法で特定の蛋白質とアイソフォームの検出と定量のためのプロトコルについて説明します。

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