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Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method
Chapters
- 00:04Title
- 01:00Sample Mix Preparation (Calculation for 150 L Sample Mix)
- 03:03Designing a New Assay Template with System Software
- 04:33Running the Assay File
- 05:20Analyze the Data
- 05:46Results: Characterization of Extracellular Signal Regulated Kinase Proteins by Capillary Isoelectric Focusing
- 07:08Conclusion
Capillary isoelectric focusing is an antibody-based, ultrasensitive, high throughput technique, enabling detailed characterization of proteins and their isoforms from extremely small biological samples. The following describes a protocol for detection and quantification of specific proteins and their isoforms in an automated and robotized manner.
