Journal
/
신경과학
/
Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

10,336 Views

•

10:35 min

•

March 15, 2018

DOI:

10.3791/56776-v

DOI:
10.3791/56776-v

•

10:35 min
March 15, 2018

•

12 Views
,
1CHU de Québec-Université Laval Research Center,Université Laval, 2Department of Biochemistry, Microbiology and Bioinformatics,Université Laval

Summary

Automatically generated

We present a method combining whole-cell patch-clamp recordings and two-photon imaging to record Ca2+ transients in neuronal dendrites in acute brain slices.

Explore More Videos

Two-photon Calcium ImagingNeuronal DendritesBrain SlicesWhole-cell Patch-clampCalcium SignalingSynaptic InputsSpatiotemporal ResolutionACSF-sucroseVibratomeHippocampal SlicesACSF-recoveryTwo-photon MicroscopeInfrared Differential Interference Contrast MicroscopyStimulating PipettePatch PipetteMultiClamp AmplifierVoltage Clamp

Read Article

Cite this Article

Camiré, O., Topolnik, L. Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices. J. Vis. Exp. (133), e56776, doi:10.3791/56776 (2018).

Download .ris file

Copy

Share Video

.

Copy