Journal
/
Neurociência
/
Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

DOI:
10.3791/56776-v

•

10:35 min

•

March 15, 2018

•

,
1CHU de Québec-Université Laval Research Center,Université Laval, 2Department of Biochemistry, Microbiology and Bioinformatics,Université Laval

Capítulos

  • 00:04Título
  • 00:46Hippocampal Slice Preparation
  • 02:03Whole-cell Patch-clamp Recordings
  • 04:33Two-photon Ca2+ Imaging
  • 08:36Results: Postsynaptic Ca2+ Transients Evoked by a Burst of Electrical Stimulation
  • 09:49Conclusion

Summary

Tadução automática

Tadução automática

We present a method combining whole-cell patch-clamp recordings and two-photon imaging to record Ca2+ transients in neuronal dendrites in acute brain slices.

Tags

Two-photon Calcium ImagingNeuronal DendritesBrain SlicesWhole-cell Patch-clampCalcium SignalingSynaptic InputsSpatiotemporal ResolutionACSF-sucroseVibratomeHippocampal SlicesACSF-recoveryTwo-photon MicroscopeInfrared Differential Interference Contrast MicroscopyStimulating PipettePatch PipetteMultiClamp AmplifierVoltage Clamp

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices

Voltage-sensitive Dye Recording from Axons, Dendrites and Dendritic Spines of Individual Neurons in Brain Slices

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

In vivo Neuronal Calcium Imaging in C. elegans

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice

Two-photon Imaging of Microglial Processes’ Attraction Toward ATP or Serotonin in Acute Brain Slices

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

In vivo Calcium Imaging in Mouse Inferior Olive

In Vivo Wide-Field and Two-Photon Calcium Imaging from a Mouse Using a Large Cranial Window

Read Article