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Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography
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생체공학
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JoVE 신문 생체공학
Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography

Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography

DOI:
10.3791/56346-v

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04:24 min

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November 22, 2017

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, ,
1Department of Kinesiology, Faculty of Applied Health Sciences,Brock University, 2Department of Health Sciences, Faculty of Applied Health Sciences,Brock University

Chapters

  • 00:05Title
  • 00:52Rat Hind Limb Positioning and Restraint for Micro-computed Tomography (microCT) Imaging
  • 02:51Results: Representative microCT Imaging of Proximal Tibia Cross-sections
  • 03:33Conclusion

Summary

자동 번역

자동 번역

This paper instructs users of in vivo micro-computed tomography (µCT) scanners how to anesthetize, correctly position and restrain the hind limb of a rat for minimal movement during high-resolution imaging of the tibia. The result is high quality images that can be processed to accurately quantify bone micro-architecture.

Tags

Rat Hind LimbBone Micro-architectureIn Vivo Micro-computed TomographyPositioningRestraintFoam TubeDental WaxMasking TapeVet WrapLongitudinal StudiesQuantification

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