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Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography
JoVE 杂志
生物工程
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JoVE 杂志 生物工程
Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography

Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography

9,596 Views

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04:24 min

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November 22, 2017

DOI:

10.3791/56346-v

DOI:
10.3791/56346-v

•

04:24 min
November 22, 2017

•

3 Views
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1Department of Kinesiology, Faculty of Applied Health Sciences,Brock University, 2Department of Health Sciences, Faculty of Applied Health Sciences,Brock University

Summary

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This paper instructs users of in vivo micro-computed tomography (µCT) scanners how to anesthetize, correctly position and restrain the hind limb of a rat for minimal movement during high-resolution imaging of the tibia. The result is high quality images that can be processed to accurately quantify bone micro-architecture.

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Cite this Article

Longo, A. B., Sacco, S. M., Ward, W. E. Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography. J. Vis. Exp. (129), e56346, doi:10.3791/56346 (2017).

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