Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using <em>In Vivo</em> Micro-computed Tomography

Amanda B. Longo; Sandra M. Sacco; Wendy E. Ward

doi:10.3791/56346

/

Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography

JoVE Journal
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JoVE Journal Biyomühendislik

Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography

Proper Positioning and Restraint of a Rat Hind Limb for Focused High Resolution Imaging of Bone Micro-architecture Using In Vivo Micro-computed Tomography

DOI:

10.3791/56346-v

•

04:24 min

•

November 22, 2017

•

Amanda B. Longo, Sandra M. Sacco, Wendy E. Ward²

¹Department of Kinesiology, Faculty of Applied Health Sciences,Brock University, ²Department of Health Sciences, Faculty of Applied Health Sciences,Brock University

Bölümler

00:05Başlık
00:52Rat Hind Limb Positioning and Restraint for Micro-computed Tomography (microCT) Imaging
02:51Results: Representative microCT Imaging of Proximal Tibia Cross-sections
03:33Conclusion

Özet

Otomatik Çeviri

This paper instructs users of in vivo micro-computed tomography (µCT) scanners how to anesthetize, correctly position and restrain the hind limb of a rat for minimal movement during high-resolution imaging of the tibia. The result is high quality images that can be processed to accurately quantify bone micro-architecture.

Etiketler

Rat Hind Limb Bone Micro-architecture In Vivo Micro-computed Tomography Positioning Restraint Foam Tube Dental Wax Masking Tape Vet Wrap Longitudinal Studies Quantification

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