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Preparación de muestras para identificación basada en espectrometría de masa de regiones de unión de ARN
1Epigenetics Institute,University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology,University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics,University of Pennsylvania Perelman School of Medicine
Chapters
- 00:05Title
- 01:04Crosslink of Protein-RNA Interactions in Live Cells
- 03:26Isolation of Nuclei
- 04:27Lysis of Nuclei and Trypsin Digestion
- 05:51Desalting of Peptides
- 07:39Removal of Crosslinked RNA
- 08:44Results: RNA-binding Protein and RNA-binding Region Identification
- 10:04Conclusion
