We present a protocol for rapid characterization of biomolecular folding and binding interactions with thermolabile ligands using differential scanning calorimetry.
Harkness V, R. W., Johnson, P. E., Mittermaier, A. K. Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions. J. Vis. Exp. (129), e55959, doi:10.3791/55959 (2017).