Journal
/
Bioquímica
/
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
JoVE Revista
Bioquímica
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Bioquímica
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

8,094 Views

•

09:15 min

•

November 21, 2017

DOI:

10.3791/55959-v

DOI:
10.3791/55959-v

•

09:15 min
November 21, 2017

•

2 Views
, ,
1Department of Chemistry,McGill University, 2Department of Chemistry,York University

Summary

Automatically generated

We present a protocol for rapid characterization of biomolecular folding and binding interactions with thermolabile ligands using differential scanning calorimetry.

Explore More Videos

Biomolecular FoldingBinding InteractionsDifferential Scanning Calorimetry (DSC)Thermolabile LigandThermodynamic ParametersBiocalorimetryDrug DesignLigand TitrationDialysisBuffer MatchingBiomolecule ConcentrationDegassingDSC Setup

Read Article

Cite this Article

Harkness V, R. W., Johnson, P. E., Mittermaier, A. K. Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions. J. Vis. Exp. (129), e55959, doi:10.3791/55959 (2017).

Download .ris file

Copy

Share Video

.

Copy