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Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
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Biochimica
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JoVE Journal Biochimica
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

DOI:
10.3791/55959-v

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09:15 min

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November 21, 2017

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, ,
1Department of Chemistry,McGill University, 2Department of Chemistry,York University

Capitoli

  • 00:05Titolo
  • 00:44Sample Preparation
  • 02:11DSC Preparation
  • 05:32Collecting Thermolabile Ligand DSC Datasets
  • 07:39Results: Rapid Characterization of Biomolecular Folding and Binding Interactions with Thermolabile Ligands by DSC
  • 08:21Conclusion

Summary

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We present a protocol for rapid characterization of biomolecular folding and binding interactions with thermolabile ligands using differential scanning calorimetry.

Tags

Biomolecular FoldingBinding InteractionsDifferential Scanning Calorimetry (DSC)Thermolabile LigandThermodynamic ParametersBiocalorimetryDrug DesignLigand TitrationDialysisBuffer MatchingBiomolecule ConcentrationDegassingDSC Setup

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