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Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions
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Biochimica
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JoVE Journal Biochimica
Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

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09:15 min

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November 21, 2017

DOI:

10.3791/55959-v

DOI:
10.3791/55959-v

•

09:15 min
November 21, 2017

•

2 Views
, ,
1Department of Chemistry,McGill University, 2Department of Chemistry,York University

Summary

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We present a protocol for rapid characterization of biomolecular folding and binding interactions with thermolabile ligands using differential scanning calorimetry.

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Biomolecular FoldingBinding InteractionsDifferential Scanning Calorimetry (DSC)Thermolabile LigandThermodynamic ParametersBiocalorimetryDrug DesignLigand TitrationDialysisBuffer MatchingBiomolecule ConcentrationDegassingDSC Setup

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Cite this Article

Harkness V, R. W., Johnson, P. E., Mittermaier, A. K. Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions. J. Vis. Exp. (129), e55959, doi:10.3791/55959 (2017).

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