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Imunocoloración Cuádruple del Bulbo Olfativo para la Visualización de Códigos de Identidad Molecular del Axón Sensorial Olfativo
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Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
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Imunocoloración Cuádruple del Bulbo Olfativo para la Visualización de Códigos de Identidad Molecular del Axón Sensorial Olfativo

6,978 Views

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06:32 min

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June 05, 2017

DOI:

10.3791/55893-v

DOI:
10.3791/55893-v

•

06:32 min
June 05, 2017

•

7 Views
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1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Center for Information and Neural Networks,National Institute of Information and Communications Technology, 3PRESTO,Japan Science and Technology Agency (JST)

Summary

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Las neuronas sensoriales olfativas expresan una amplia variedad de moléculas de clasificación de axones para establecer circuitos neurales adecuados. Este protocolo describe un método de tinción inmunohistoquímica para visualizar las expresiones combinatorias de las moléculas de clasificación de axones en los terminales axónicos de las neuronas sensoriales olfatorias.

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Cite this Article

Ihara, N., Ikegaya, Y., Takeuchi, H. Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes. J. Vis. Exp. (124), e55893, doi:10.3791/55893 (2017).

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