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Quadruple Immunfärbung der olfaktorischen Glühbirne zur Visualisierung von olfaktorischen sensorischen Axon Molecular Identity Codes
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Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes
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Quadruple Immunfärbung der olfaktorischen Glühbirne zur Visualisierung von olfaktorischen sensorischen Axon Molecular Identity Codes

6,978 Views

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06:32 min

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June 05, 2017

DOI:

10.3791/55893-v

DOI:
10.3791/55893-v

•

06:32 min
June 05, 2017

•

7 Views
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1Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences,The University of Tokyo, 2Center for Information and Neural Networks,National Institute of Information and Communications Technology, 3PRESTO,Japan Science and Technology Agency (JST)

Summary

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Olfaktorische sensorische Neuronen exprimieren eine Vielzahl von Axon-sortierenden Molekülen, um eine korrekte neuronale Schaltung zu etablieren. Dieses Protokoll beschreibt eine immunhistochemische Färbemethode, um kombinatorische Ausdrücke von Axon-sortierenden Molekülen an den Axon-Termini von olfaktorischen sensorischen Neuronen zu visualisieren.

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Ihara, N., Ikegaya, Y., Takeuchi, H. Quadruple Immunostaining of the Olfactory Bulb for Visualization of Olfactory Sensory Axon Molecular Identity Codes. J. Vis. Exp. (124), e55893, doi:10.3791/55893 (2017).

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