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Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
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June 16, 2017
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This work describes an optimized methyl-CpG-binding domain (MBD) sequencing protocol and a computational pipeline to identify differentially methylated CpG-rich regions in chronic lymphocytic leukemia (CLL) patients.
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DNA MethylationMethyl-CpG-binding DomainCapture-based MethodChronic Lymphocytic LeukemiaNext-generation SequencingGlobal Methylation ProfilingDifferentially Methylated CpG-rich RegionsCLL-specific Signature GenesDisease PathogenesisPrognosisGenomic DNA ExtractionSonicationMagnetic Streptavidin BeadsMBD-biotin Protein
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Subhash, S., Kanduri, M. Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients. J. Vis. Exp. (124), e55773, doi:10.3791/55773 (2017).
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