Journal
/
유전학
/
Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay

4,854 Views

•

10:32 min

•

February 03, 2022

DOI:

10.3791/63448-v

DOI:
10.3791/63448-v

•

10:32 min
February 03, 2022

•

28 Views
, , ,
1Department of Microbiology, Institute of Biomedical Sciences,University of São Paulo, 2Division of Oncology, Department of Internal Medicine,Washington University in St. Louis

Summary

Automatically generated

Here we describe two modifications of the DNA fiber assay to investigate single-stranded DNA gaps in replicating DNA after lesion induction. The S1 fiber assay enables the detection of post-replicative gaps using the ssDNA-specific S1 endonuclease, while the gap-filling assay allows visualization and quantification of gap repair.

Explore More Videos

DNA Fiber AssayPost-replicative GapsS1 NucleaseDNA RepairReplication StressGenome IntegrityUV-C IrradiationCldU IncorporationCSK PermeabilizationDNA Lesion Detection

Read Article

Cite this Article

Martins, D. J., Tirman, S., Quinet, A., Menck, C. F. M. Detection of Post-Replicative Gaps Accumulation and Repair in Human Cells Using the DNA Fiber Assay. J. Vis. Exp. (180), e63448, doi:10.3791/63448 (2022).

Download .ris file

Copy

Share Video

.

Copy