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Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae
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Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae

2,610 Views

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09:25 min

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June 02, 2021

DOI:

10.3791/62423-v

DOI:
10.3791/62423-v

•

09:25 min
June 02, 2021

•

2 Views
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1Beijing Key Laboratory of New Technology in Agricultural Application, National Demonstration Center for Experimental Plant Production Education, Department of Agronomy,Beijing University of Agriculture

Summary

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Here, we present a protocol to analyze the genome-wide distribution of histone modifications, which can identify new target genes in the pathogenesis of M. oryzae and other filamentous fungi.

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Chromatin Immunoprecipitation Sequencing (ChIP-seq)Histone ModificationsMagnaporthe OryzaeEpigenetic RegulationFungal PathogenesisGenome-wide AnalysisTranscription FactorsDNA-protein InteractionsProtoplast PreparationSonicationAgarose Gel Electrophoresis

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Cite this Article

Wu, Z., Sun, W., Zhou, S., Zhang, L., Zhao, X., Xu, Y., Wang, W. Genome-wide Analysis of Histone Modifications Distribution using the Chromatin Immunoprecipitation Sequencing Method in Magnaporthe oryzae. J. Vis. Exp. (172), e62423, doi:10.3791/62423 (2021).

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