Journal
/
생화학
/
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

9,829 Views

•

10:23 min

•

April 17, 2017

DOI:

10.3791/55585-v

DOI:
10.3791/55585-v

•

10:23 min
April 17, 2017

•

16 Views
, , ,
1Heinrich Pette Institute,Leibniz Institute for Experimental Virology, 2Core Facility Mass Spectrometric Proteomics,University Medical Center Hamburg-Eppendorf

Summary

Automatically generated

Lipid droplets are important organelles for the replication of several pathogens, including the Hepatitis C Virus (HCV). We describe a method to isolate lipid droplets for quantitative mass spectrometry of associated proteins; it can be used under a variety of conditions, such as virus infection, environmental stress, or drug treatment.

Explore More Videos

Lipid Droplet IsolationMass Spectrometry AnalysisQuantitative AnalysisHepatitis C VirusSILAC MediumHeavy And Light Labeled CellsProtein IncorporationSDS-PAGEMS Analysis

Read Article

Cite this Article

Rösch, K., Kwiatkowski, M., Schlüter, H., Herker, E. Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis. J. Vis. Exp. (122), e55585, doi:10.3791/55585 (2017).

Download .ris file

Copy

Share Video

.

Copy