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Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
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April 17, 2017
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Lipid droplets are important organelles for the replication of several pathogens, including the Hepatitis C Virus (HCV). We describe a method to isolate lipid droplets for quantitative mass spectrometry of associated proteins; it can be used under a variety of conditions, such as virus infection, environmental stress, or drug treatment.
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Rösch, K., Kwiatkowski, M., Schlüter, H., Herker, E. Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis. J. Vis. Exp. (122), e55585, doi:10.3791/55585 (2017).
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