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In vivo beeldvorming van transgene Gene Expression Individuele retinale voorouders in chimère zebravis embryo's to Cell Nonautonomous Invloeden Studie
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In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
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In vivo beeldvorming van transgene Gene Expression Individuele retinale voorouders in chimère zebravis embryo's to Cell Nonautonomous Invloeden Studie

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10:36 min

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March 22, 2017

DOI:

10.3791/55490-v

DOI:
10.3791/55490-v

•

10:36 min
March 22, 2017

•

2 Views
, , ,
1School of Biosciences,The University of Melbourne, 2Australian Regenerative Medicine Institute (ARMI),Monash University, 3The David J Apple Center for Vision Research, Department of Ophthalmology,Heidelberg University

Summary

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Live volgen van individuele WT retinale voorlopers in verschillende genetische achtergronden maakt voor de beoordeling van de bijdrage van de cel niet-zelfstandige signalering tijdens de neurogenese. Hier, een combinatie van gen knockdown, chimeer generatie via embryo transplantatie en in vivo time-lapse confocale beeldvorming werd gebruikt voor dit doel.

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Cite this Article

Dudczig, S., Currie, P. D., Poggi, L., Jusuf, P. R. In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences. J. Vis. Exp. (121), e55490, doi:10.3791/55490 (2017).

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