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Developmental Biology

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JoVE Journal Developmental Biology
In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
 

In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences

Article DOI: 10.3791/55490-v • 10:36 min • March 22nd, 2017
March 22nd, 2017 •
1,2, 2, 3, 1,2
1School of Biosciences, The University of Melbourne, 2Australian Regenerative Medicine Institute (ARMI), Monash University, 3The David J Apple Center for Vision Research, Department of Ophthalmology, Heidelberg University

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Live tracking of individual WT retinal progenitors in distinct genetic backgrounds allows for the assessment of the contribution of cell non-autonomous signaling during neurogenesis. Here, a combination of gene knockdown, chimera generation via embryo transplantation and in vivo time-lapse confocal imaging was utilized for this purpose.

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Keywords: In Vivo Imaging Transgenic Gene Expression Retinal Progenitor Cells Chimeric Zebrafish Embryos Cell Non-autonomous Influences Fate Specification Microinjection H2AGFP H2BRFP MRNA Fluorescence Microscopy
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