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In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
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In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences

In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences

7,569 Views

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10:36 min

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March 22, 2017

DOI:

10.3791/55490-v

DOI:
10.3791/55490-v

•

10:36 min
March 22, 2017

•

2 Views
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1School of Biosciences,The University of Melbourne, 2Australian Regenerative Medicine Institute (ARMI),Monash University, 3The David J Apple Center for Vision Research, Department of Ophthalmology,Heidelberg University

Summary

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Live tracking of individual WT retinal progenitors in distinct genetic backgrounds allows for the assessment of the contribution of cell non-autonomous signaling during neurogenesis. Here, a combination of gene knockdown, chimera generation via embryo transplantation and in vivo time-lapse confocal imaging was utilized for this purpose.

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In Vivo ImagingTransgenic Gene ExpressionRetinal Progenitor CellsChimeric Zebrafish EmbryosCell Non-autonomous InfluencesFate SpecificationMicroinjectionH2AGFPH2BRFPMRNAFluorescence Microscopy

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Dudczig, S., Currie, P. D., Poggi, L., Jusuf, P. R. In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences. J. Vis. Exp. (121), e55490, doi:10.3791/55490 (2017).

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