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In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
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JoVE Journal 発生生物学
In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences

In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences

DOI:
10.3791/55490-v

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10:36 min

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March 22, 2017

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, , ,
1School of Biosciences,The University of Melbourne, 2Australian Regenerative Medicine Institute (ARMI),Monash University, 3The David J Apple Center for Vision Research, Department of Ophthalmology,Heidelberg University

章

  • 00:05標題
  • 00:52Embryo Microinjection
  • 02:53Preparation for Transplantation
  • 04:59Chimera Generation via Blastula Transplantation
  • 06:37Mounting for Live Imaging
  • 08:27Results: Transgene Expression in Retinal Progenitors from Donor Cells
  • 09:34Conclusion

概要

自動翻訳

自動翻訳

Live tracking of individual WT retinal progenitors in distinct genetic backgrounds allows for the assessment of the contribution of cell non-autonomous signaling during neurogenesis. Here, a combination of gene knockdown, chimera generation via embryo transplantation and in vivo time-lapse confocal imaging was utilized for this purpose.

タグ

In Vivo ImagingTransgenic Gene ExpressionRetinal Progenitor CellsChimeric Zebrafish EmbryosCell Non-autonomous InfluencesFate SpecificationMicroinjectionH2AGFPH2BRFPMRNAFluorescence Microscopy

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