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Using Primary Neurosphere Cultures to Study Primary Cilia
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April 14, 2017
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The primary cilium is fundamentally important in neural progenitor cell proliferation, neuronal differentiation, and adult neuronal function. Here, we describe a method to study ciliogenesis and the trafficking of signaling proteins to cilia in neural stem/progenitor cells and differentiated neurons using primary neurosphere cultures.
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Shimada, I. S., Badgandi, H., Somatilaka, B. N., Mukhopadhyay, S. Using Primary Neurosphere Cultures to Study Primary Cilia. J. Vis. Exp. (122), e55315, doi:10.3791/55315 (2017).
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