Using Primary Neurosphere Cultures to Study Primary Cilia

Issei S. Shimada; Hemant Badgandi; Bandarigoda N. Somatilaka; Saikat Mukhopadhyay

doi:10.3791/55315

ジャーナル

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発生生物学

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Using Primary Neurosphere Cultures to Study Primary Cilia

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発生生物学

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JoVE Journal 発生生物学

Using Primary Neurosphere Cultures to Study Primary Cilia

9,189 Views

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08:14 min

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April 14, 2017

DOI:

10.3791/55315-v

DOI:

10.3791/55315-v

•

08:14 min

April 14, 2017

•

6 Views

Issei S. Shimada¹, Hemant Badgandi¹, Bandarigoda N. Somatilaka¹, Saikat Mukhopadhyay¹

¹Department of Cell Biology,University of Texas Southwestern Medical Center

概要

The primary cilium is fundamentally important in neural progenitor cell proliferation, neuronal differentiation, and adult neuronal function. Here, we describe a method to study ciliogenesis and the trafficking of signaling proteins to cilia in neural stem/progenitor cells and differentiated neurons using primary neurosphere cultures.

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Using Primary Neurosphere Cultures to Study Primary Cilia

概要

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