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Developmental Biology

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JoVE Journal Developmental Biology
Using Primary Neurosphere Cultures to Study Primary Cilia
 

Using Primary Neurosphere Cultures to Study Primary Cilia

Article DOI: 10.3791/55315-v • 08:14 min • April 14th, 2017
April 14th, 2017 •
1, 1, 1, 1
1Department of Cell Biology, University of Texas Southwestern Medical Center

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The primary cilium is fundamentally important in neural progenitor cell proliferation, neuronal differentiation, and adult neuronal function. Here, we describe a method to study ciliogenesis and the trafficking of signaling proteins to cilia in neural stem/progenitor cells and differentiated neurons using primary neurosphere cultures.

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Primary Neurosphere Cultures Primary Cilia Neurosphere-based Culturing Methods Primary Neural Stem Cells Neural Progenitors Neurons Cilia Biogenesis Trafficking Of Cilia Molecules Primary Cell Criteria Biogenesis And Ciliary Pathways Culturing Neural Stem Cells Lateral Ventricle Dissection Whole Mount Dissection Method Trypsin-EDTA Stopping Medium
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