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Single-celle Gene Expression Profiling Brug FACS og qPCR med interne standarder
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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Single-celle Gene Expression Profiling Brug FACS og qPCR med interne standarder

16,155 Views

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10:50 min

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February 25, 2017

DOI:

10.3791/55219-v

DOI:
10.3791/55219-v

•

10:50 min
February 25, 2017

•

9 Views
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1Laboratory of Pathology, Center for Cancer Research,National Cancer Institute, 2Experimental Transplantation and Immunology Branch, Center for Cancer Research,National Cancer Institute

Summary

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We describe a method to sort single mammalian cells and to quantify the expression of up to 96 target genes of interest in each cell. This method includes the use of internal qPCR standards to enable the estimation of absolute transcript counts.

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Porter, J. R., Telford, W. G., Batchelor, E. Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards. J. Vis. Exp. (120), e55219, doi:10.3791/55219 (2017).

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