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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
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JoVE Journal Genetik
Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

16,166 Views

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10:50 min

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February 25, 2017

DOI:

10.3791/55219-v

DOI:
10.3791/55219-v

•

10:50 min
February 25, 2017

•

9 Views
, ,
1Laboratory of Pathology, Center for Cancer Research,National Cancer Institute, 2Experimental Transplantation and Immunology Branch, Center for Cancer Research,National Cancer Institute

Özet

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We describe a method to sort single mammalian cells and to quantify the expression of up to 96 target genes of interest in each cell. This method includes the use of internal qPCR standards to enable the estimation of absolute transcript counts.

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Porter, J. R., Telford, W. G., Batchelor, E. Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards. J. Vis. Exp. (120), e55219, doi:10.3791/55219 (2017).

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